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generation lentivirus particles  (TaKaRa)


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  • 99

    Structured Review

    TaKaRa generation lentivirus particles
    Generation Lentivirus Particles, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+lentivirus+particles/Lenti-X+Concentrator/pmc04861406-57-12-15
    Average 99 stars, based on 4634 article reviews
    generation lentivirus particles - by Bioz Stars, 2026-09
    99/100 stars

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    shRNA:

    Article Title: Endoplasmic Reticulum-resident Heat Shock Protein 90 (HSP90) Isoform Glucose-regulated Protein 94 (GRP94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport
    Article Snippet: Cell Culture and Stable Cell Lines The GRP94 shRNA construct along with the control (nonspecific shRNA) were obtained as glycerol stocks, and the plasmids were recovered with a Qiagen plasmid kit (MIDI prep) using the manufacturer's protocol. .. The plasmids containing the nonspecific and GRP94-specific shRNA were packaged into third generation lentivirus particles (Clontech) and concentrated using the Lenti-X concentrator kit. .. The concentrated lentiviral particles were transduced according to the protocol mentioned in the Open Biosystems manual and then selected for puromycin resistance.

    Article Title: Endoplasmic Reticulum-resident Heat Shock Protein 90 (HSP90) Isoform Glucose-regulated Protein 94 (GRP94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport
    Article Snippet: Cell culture and stable cell lines The Grp94 shRNA construct along with the control (nonspecific shRNA) were obtained as glycerol stocks, and the plasmids recovered with a Qiagen Plasmid kit (MIDI prep) using the by guest on M arch 9, 2016 http://w w w .jbc.org/ D ow nloaded from 3 manufacturer’s protocol. .. The plasmids containing the nonspecific and Grp94 specific shRNA were packaged into third generation lentivirus particles (Clontech, Mountain View, CA) and concentrated using the Lenti-X concentrator kit. .. The concentrated lentiviral particles were transduced according to the protocol mentioned in the Open Biosystems manual and then selected for puromycin resistance.



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